Journal: Scientific Reports

Article Title: GCC2 promotes non-small cell lung cancer progression by maintaining Golgi apparatus integrity and stimulating EGFR signaling pathways

doi: 10.1038/s41598-024-75316-1

Figure Lengend Snippet: GCC2 knockdown inhibits NSCLC proliferation, migration, invasion, and survival. ( A ) RT-qPCR analysis and ( B ) western blot analysis of relative GCC2 expression levels in H460 cells following transfection with shGCC2. ( C ) Cell viabilities of GCC2 knockdown H460 cells were measured by MTS assay at 0, 4, 8, 12, and 24 h. ( D ) Detection of TUNEL-positive cells in H460 cells transfected with shGCC2 plasmids. Scale bar = 50 μm. RT-qPCR analysis of relative gene expression of ( E ) CSC-related biomarkers and ( F ) EMT-related genes. ( G ) Colony formation assays of control and GCC2 knockdown H460 cells with colony counts summarized on the right. ( H ) Wound-healing assay measured at 0 and 48 h demonstrating the migratory potential of control and GCC2 knockdown H460 cells. Scale bar = 100 μm. ( I ) Transwell migration and invasion assays in control and GCC2 knockdown H460 cells. Scale bar = 100 μm. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Cell migration and invasion assays were performed using Transwell chambers with 8 um pore polycarbonate membrane inserts (Corning, NY, USA).

Techniques: Knockdown, Migration, Quantitative RT-PCR, Western Blot, Expressing, Transfection, MTS Assay, TUNEL Assay, Gene Expression, Control, Wound Healing Assay

Journal: Scientific Reports

Article Title: GCC2 promotes non-small cell lung cancer progression by maintaining Golgi apparatus integrity and stimulating EGFR signaling pathways

doi: 10.1038/s41598-024-75316-1

Figure Lengend Snippet: GCC2 knockdown decreases exosome release and abolishes the migration- and proliferation-promoting effects of cancer cell-derived exosomes. ( A ) Total number of exosomes released in control and GCC2 knockdown H460 cells were determined by nanoparticle tracking analysis. ( B ) Immunofluorescence results of CD63-positive exosomes (green) in H460 and H1299 cells. Cell nuclei were stained with Hoechst. Scale bar = 10 μm. H460 cell-derived, GCC2 + exosomes were collected and measured by nanoparticle tracking analysis, and H460 cells were treated with GCC2 + exosomes in a dose-dependent manner for 48 h. ( C ) Heat-map indicating the changes in mRNA expression levels of CSC- and EMT-related genes of H460 cells treated with GCC2 + exosomes. ( D ) Transwell migration and invasion assays were performed to assess the migratory ability of H460 cells treated with GCC2 + exosomes. Scale bar = 100 μm. ( E ) Simultaneously, colony formation assays were performed in H460 cells treated with GCC2 + exosomes. GCC2 knockdown H460 cell-derived, GCC2 KD exosomes were isolated and treated on H460 cells in a dose-dependent manner. ( F ) Heat-map indicating changes in mRNA expression levels of CSC- and EMT-related genes in H460 cells treated with GCC2 KD exosomes. ( G ) Transwell migration and invasion assays were performed to assess the migratory ability of H460 cells following GCC2 KD exosome treatment. Scale bar = 100 μm. ( H ) Colony formation abilities of H460 cells treated with GCC2 KD exosomes. ( I ) Graphs comparing results of migration, invasion, and colony formation assays of H460 cells treated with either GCC2 + or GCC2 KD exosomes in increasing doses. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Cell migration and invasion assays were performed using Transwell chambers with 8 um pore polycarbonate membrane inserts (Corning, NY, USA).

Techniques: Knockdown, Migration, Derivative Assay, Control, Immunofluorescence, Staining, Expressing, Isolation

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Cancer-Associated Fibroblasts Autophagy Enhances Progression of Triple-Negative Breast Cancer Cells

doi: 10.12659/MSM.902870

Figure Lengend Snippet: TNBC cell lines migration and invasion analysis. ( A ) TNBC cells migration was detected by Matrigel-coated Transwell. ( B ) The comparison of migration for different groups of TNBC cells. ( C ) The comparison of invasion for different groups of TNBC cells. * P <0.05.

Article Snippet: For migration and invasion assay, 8-uM pore size Transwell inserts (BD Biosciences, USA) were used.

Techniques: Migration